Kungani i-365nm LED Ibalulekile Ezinhlelweni Ezisebenzayo Ze-Fluorescence?

Izicelo ze-fluorescence seziphenduke izinsika ezizindeni eziningi ezahlukene zesayensi nezimboni njengoba zinikeza ukutholwa kwamangqamuzana okunembile kanye nokubonwa. Ukuthi noma umuntu uphenya izimfihlo zebhayoloji yamaselula noma uthola ubufakazi obufihlekile bomthetho, ikhwalithi yomthombo wokukhanya osetshenzisiwe inquma ukusebenza kahle kwalokhu kusetshenziswa.

Manje, faka i-365nm LED, uguquko oluguquguqukayo kubuchwepheshe be-fluorescence. Lawa ma-LED akhiqiza ukukhanya kubude begagasi obufanele bokuvuselela i-spectrum ebanzi ye-fluorophore, akhiqize izithombe ezicace kakhudlwana kanye nedatha enembe kakhudlwana.

Nokho, yini ehlukanisa i-365 nm UV LED phakathi kolwandle lwemithombo yokukhanya? Lesi sihloko sizophenya ngezinzuzo zabo ezimbalwa futhi sixoxe ngokuthi kungani kudingekile ekusetshenzisweni kwe-fluorescence. I-Tianhui UV LED iwumthombo omuhle kakhulu wemikhiqizo ye-LED ye-premium engu-365nm UV.

Yini i-Fluorescence

I-fluorescence inqubo lapho amanye ama-molecule amunca ukukhanya kubude obubodwa be-wavelength abese ekukhipha kwenye, ngokuvamile ebangeni elide. Le khwalithi isetshenziswa kakhulu ukuze kubukwe, kukhonjwe, futhi kulinganise ama-molecule athile ezizindeni zesayensi, zezokwelapha, nezimboni.

Ukuqapha indawo ezungezile, ukuhlaziya amakhemikhali, kanye ne-biological imaging kuzuza kakhulu ku-fluorescence. Ithola ngisho namaleveli amaminithi ezidakamizwa kumasampuli ayinkimbinkimbi, ihlinzeka ngokuzwela okukhulu nokucaciswa. Ukukhishwa kokukhanya okuqondile kwama-fluorophores kuvumela abacwaningi ukuthi bahlole amaseli, ubuthi bamabala, futhi bahlole ngokunembile amasampula ebhayoloji.

Iqhaza lama-LED angu-365nm

Izicelo ku-fluorescence zincike kakhulu ekukhanyeni kwe-Led engu-365 nm. Lezi izindima abazinikezayo, ngakho zibalulekile emikhakheni eminingi.

1  I-Excitation Elungile ye-Fluorophores

Ukuze kujabulise ama-fluorophoreson amaningi avamile, i-365nm UV LED ikhiqiza ukukhanya kwe-UV ngobude obuwusizo impela. Le wavelength ilungele ukukhiqiza la ma-molecule ukuze akhanye, akhiqize isignali eqinile necacile. Njengoba enikeza amandla ajabulisayo adingekayo ukuze kukhuliswe amandla e-fluorescence, ama-LED angu-365 nm aphakeme kuneminye imithombo yokukhanya, okuhlanganisa i-fluorescence microscopy ne-spectroscopy.

2  Ukuzwela Okuphezulu kanye Nokulungiswa

Ukuqina okukhulu kanye nokuzinza kwama-LED angu-365 nm kunika amandla ukusetshenziswa kwe-fluorescence ngokuzwela nokulungiswa. Ucwaningo luncike emiphumeleni enembile nephindaphindwayo, ethembele ekuphumeni kokukhanya okungaguquki, ngakho lawa ma-LED abalulekile.

Ezilungiselelweni zocwaningo nezokuxilonga, kanye nasezithombeni ezibanzi, zilungele ukukalwa ngokunemba. Ama-LED we-Tianhui UV imikhiqizo yenzelwe ukwanelisa indlela engcono kakhulu yokunemba kanye nekhwalithi yalabo abafuna okuyinkimbinkimbi Izixazululo ze-UV LED

3  Isilinganiso esithuthukisiwe se-Signal-to-Noise

Kuzinhlelo zokusebenza ze-fluorescence, ukuhlukanisa isignali ye-fluorescence emsindweni wangemuva kuncike esignali-to-ratio ephezulu. I-365nm UV LED inika umthombo onamandla ovusa amadlingozi okhiqiza isignali ehlukile necacile ye-fluorescence. Leli khono liwusizo kakhulu kumasampuli ayinkimbinkimbi lapho kungaba inselele khona ukuthola isignali eqondiwe.

4  Ukuqina Nokwethenjelwa

Kuyaziwa ukuthi ukukhanya kwe-365nm Led yinde futhi kuhlala isikhathi eside kangakanani. Ngokungafani nemithombo yokukhanya evamile, ewohloka ngokushesha ngokuhamba kwesikhathi, ithembisa ukusebenza okuqhubekayo futhi inciphisa isidingo sokushintshwa njalo. Izinhlelo zokusebenza zesikhathi eside ezifana nokuqapha kwemvelo okuqhubekayo noma ukusebenza komkhakha okuzenzakalelayo kuncike kulokhu kwethembeka.  

Izicelo ze-365nm UV LED

Lezi ezinye zezindawo ezibalulekile lapho la ma-LED ebaluleke khona:

1  I-Fluorescence Microscopy

I-Fluorescence microscopy iphenya futhi ibuke ukwakheka kwamaselula namamolekyuli ebhayoloji kusetshenziswa izibani ezingama-365nm kakhulu. Ubude bamaza obunembile buvumela umuntu ukuthi asebenzise ama-fluorophore amaningana, akhiqize izithombe ezibukhali nezinemininingwane edingekayo ocwaningweni lwe-biomedical kanye nokuxilongwa. Buka Ama-LED we-Tianhui UV  imikhiqizo yama-LED asebenza kahle kakhulu ifanele i-microscopy.

2  Ukuhlaziywa Kwe-DNA kanye Namaprotheni

Ibhayoloji yamangqamuzana isebenzisa ama-LED angu-365 nm ukuze ifake odayi be-fluorescence ababoshelwe ku-DNA noma kumaprotheni. Izindlela zihlanganisa i-electrophoresis yejeli nama-microarrays, lapho ukuhlonza okunembayo kwe-biomolecule kanye nomthamo kuyadingeka, kuye ngayo.

3  I-Forensic Analysis

I-365 nm imele ama-LED, abalulekile ekuphenyweni kwe-forensic ekuboneni iminonjana yezinto eziphilayo—njengegazi noma olunye uketshezi lomzimba—okuyinto ekhanyayo ngaphansi kokukhanya kwe-UV. Kwenza kube lula ukuseshwa nokuhlaziywa kobufakazi ezindaweni zobugebengu—ngisho nasezimweni ezinzima.

4  Ukuqapha Imvelo

Ukuqapha imvelo kusebenzisa i-365nm UV LED ukuze ikhanye ngaphansi kobuthi bokukhanya kwe-UV kanye nokuhlonza ukungcola. Ibhekana nokulawulwa kwekhwalithi yamanzi, ukutholwa kwamakhemikhali, nokulandelelwa kokuchitheka kukawoyela. Ngenxa yokwethenjelwa kwawo, ukuzwela ezimeni zezulu eziningi, nokuqina, izibani ze-365nm Led zilungele lezi zinhloso.

Izindlela Ezingemuva kwama-LED angu-365nm

Ukuthuthukisa ama-LED angu-365 nm kudinga ukuqonda imigomo yawo eyisisekelo. Phakathi kwalezi zici eziphawulekayo:

·  I-Emission Spectrum

Ama-LED angu-365 nm abonisa isasasa eliphakeme lama-fluorophore ahlukene futhi akhipha ukukhanya ngobude obuthile. I-fluorescence engcono kakhulu itholakala ekudluliselweni kwamandla okusebenzayo okuqinisekiswe yile spectrum ekhishwayo. Ukunciphisa ukuphazamiseka okuvela kwamanye amaza ngamaza ngebhendi yokukhipha okulinganiselwe kusiza ukucacisa isignali ye-fluorescence.

·  Amandla Okukhanya Nokugxila

Ukukhanya okunamandla kwama-LED angu-365 nm kuqinisekisa ukusebenza ngempumelelo kwama-fluorophores kanye nokungena okujulile, ngisho nasezintweni eziminyene noma eziwugqinsi. Ngaphezu kwalokho, ku-microscopy nakwezinye izinhlelo zokusebenza ze-imaging, ukukhanya okwenziwe ngokwezifiso okwenziwe kutholakale yilokhu Imodyuli ye-UV LED kunenzuzo.

·  Ukuphathwa Kokushisa

Ngokusiza ekunciphiseni ukushisa ngokweqile kanye nobuchwepheshe obuthuthukisiwe bokuphatha ukushisa ku-365 nm, ama-LED asiza ukuvimbela ukuwohloka kwe-LED futhi athinte ukusebenza. Ukukhipha ukushisa okulungile kuqinisekisa ukukhiqizwa kokukhanya okuqhubekayo futhi kuthuthukisa ukuphila kwe-LED, okuqinisekisa ukuthembeka kokusetshenziswa isikhathi eside ezinhlelweni ezifunwayo.  

Izinzuzo Ngaphezu Kweminye Imithombo Yokukhanya

Izinzuzo eziyinhloko ezibalwe lapha zisiza ukuchaza ukuthi kungani umuntu ethanda izinhlelo ezithile ze-fluorescence:

·  Okuphumayo Kwe-Wavelength ethize

Ngokungafani nemithombo yokukhanya evamile, njengamalambu e-mercury, ama-LED angu-365 nm UV akhiqiza ukukhanya ngobude obuthile obucacisiwe ngaphandle kwezihlungi ezengeziwe. Inikeza indlela ecacile nesebenzayo yokuvusa amadlingozi futhi yenza kube lula ukusetshenziswa kwe-fluorescence, ithuthukise ukusebenza kahle. Zitholele imikhiqizo I-Tianhui LED  Izipesheli zama-LED we-premium 365nm enzelwe ukusetshenziswa okuthile.

·  Ukusebenza Kwamandla

Enye indlela eyonga amandla njengoba ama-LED angu-365 nm adla ugesi omncane kakhulu kunemithombo yokukhanya evamile. Kushibhile futhi kunobungane emvelweni, kunciphisa wonke amandla asetshenziswa abasebenzisi be-fluorescent.

·  Usayizi Ohlangene

Kusukela kumathuluzi aselabhorethri ayinkimbinkimbi kuya kumathuluzi aphathekayo e-forensic, usayizi omncane we-365nm Led light uwavumela ukuthi afakwe kumadivayisi nezinhlelo eziningi. Umkhondo wabo ophansi awuphazamisi ukusebenza; ngakho-ke, zivumelana nezisetshenziswa eziningi ezisekelwe ku-fluorescence.

Isiphetho:

Sengiphetha, ngenxa yokuzwela kwawo okukhulu, ukuphila isikhathi eside, kanye nezimfanelo ezingcono kakhulu zokuvusa amadlingozi, i-365nm UV LED ibalulekile ekusetshenzisweni kwe-fluorescence. Zingcono kunemithombo yokukhanya evamile mayelana nokuphumayo kwe-wavelength kanye nomnotho wamandla.

Ifomu labo elincane futhi lenza ukuhlanganiswa okuguquguqukayo kumadivayisi amaningi nezinhlelo kungenzeka. Tianhuiu UV LED i-diode ihlinzeka ngohlu oluphelele lwezixazululo eziyinkimbinkimbi ukuze unelise zonke izidingo zakho ze-premium 365 nm LED imikhiqizo ehlinzekelwe ukusetshenziswa okuhlukile.