No ke aha he 365nm LED e pono ai no nā noi Fluorescence kūpono?

Ua lilo nā noi Fluorescence i mau kia i nā ʻenehana ʻepekema a me nā ʻenehana like ʻole mai ka hāʻawi ʻana i ka ʻike molekala pololei a me ka ʻike. Ke noiʻi nei kekahi i nā mea huna o ka biology cellular a i ʻole ka loaʻa ʻana o nā hōʻike forensic latent, ʻo ka maikaʻi o ke kumu kukui i hoʻohana ʻia e hoʻoholo i ka pono o kēia mau hoʻohana.

I kēia manawa, e hoʻokomo i ka 365nm LED, kahi hoʻololi hou i ka ʻenehana fluorescence. Hoʻopuka kēia mau LED i ka mālamalama i ka lōʻihi hawewe kūpono no ka hoʻoulu ʻana i kahi ākea ākea o nā fluorophores, e hana ana i nā kiʻi ʻoi aku ka maopopo a me ka ʻikepili pololei.

Eia naʻe, he aha ka mea e hoʻokaʻawale ai i ka 365 nm UV LED ma waena o ke kai o nā kumu kukui? E noiʻi kēia ʻatikala i kā lākou mau pono a kūkākūkā i ke kumu e pono ai lākou no ka hoʻohana ʻana i ka fluorescence. Tianhui UV LED He kumu maikaʻi loa ia no nā huahana 365nm UV LED.

He aha ka Fluorescence

ʻO ka Fluorescence ke kaʻina hana e hoʻomoʻa ai kekahi mau molekala i ka mālamalama ma kahi lōʻihi hawewe a laila hoʻokuʻu iā ia i kekahi, pinepine i ka nalu lōʻihi. Hoʻohana nui ʻia kēia ʻano no ka nānā ʻana, ʻike, a me ka helu ʻana i kekahi mau mole ma nā ʻaoʻao ʻepekema, lāʻau lapaʻau, a me nā ʻoihana.

ʻO ka nānā ʻana i ke kaiapuni, ka nānā ʻana i nā kemika, a me nā kiʻi biological ka pōmaikaʻi loa mai ka fluorescence. ʻIke ʻo ia i nā pae minuke o nā lāʻau lapaʻau i nā laʻana paʻakikī, e hāʻawi ana i ka naʻau a me ka kikoʻī. ʻO ka hoʻokuʻu ʻana o nā fluorophores e hiki ai i nā mea noiʻi ke nānā i nā cell, ʻike i nā mea ʻawaʻawa, a nānā pono i nā laʻana olaola.

ʻO ke kuleana o nā LED 365nm

ʻO nā noi i ka fluorescence e hilinaʻi nui ʻia ma 365 nm Led kukui. ʻO kēia nā kuleana a lākou e hāʻawi ai, no laila he mea nui lākou i nā kahua he nui.

1  ʻO ka hauʻoli maikaʻi loa no nā Fluorophores

No ka hauʻoli o nā fluorophoresons maʻamau, 365nm UV LED e hoʻopuka i ke kukui UV ma kahi lōʻihi hawewe pono. He mea kūpono kēia nalu nalu no ka hana ʻana i kēia mau molekala e ʻālohilohi, e hana ana i kahi hōʻailona ikaika a maopopo. No ka mea hāʻawi lākou i ka ikehu hoʻoulu e koi ʻia e hoʻonui i ka ikaika o ka fluorescence, ʻoi aku ka maikaʻi o nā LED 365 nm ma mua o nā kumu kukui ʻē aʻe, me ka microscopy fluorescence a me ka spectroscopy.

2  ʻO kaʻike kiʻekiʻe a me ka hoʻoholo

ʻO ka ikaika nui a me ka paʻa o nā LED 365 nm hiki ke hoʻohana i ka fluorescence me ka naʻau a me ka hoʻonā. Ke hilinaʻi nei nā haʻawina i nā hopena pololei a hiki ke hoʻihoʻi ʻia, e hilinaʻi nei i ka puka māmā mau, no laila he mea koʻikoʻi kēia mau LED.

Ma ka noiʻi a me nā hoʻonohonoho hōʻoia, a me nā kiʻi kikoʻī, kūpono lākou no nā ana pololei. ʻO Tianhui UV LED Hana ʻia nā huahana e hoʻokō i nā pae hoʻohālikelike maikaʻi loa o ka pololei a me ka maikaʻi no ka poʻe e ʻimi nei i ka maʻalahi Donmake aʻu aa

3  Hoʻomaikaʻi ʻia ka hōʻailona-a-noise ratio

Ma nā noi fluorescence, ʻo ka hoʻokaʻawale ʻana i ka hōʻailona fluorescence mai ka walaʻau hope e hilinaʻi ʻia i kahi hōʻailona-i-ratio kiʻekiʻe. Hāʻawi ʻo 365nm UV LED i kahi kumu hauʻoli ikaika e hana i kahi hōʻailona fluorescence ʻokoʻa a maopopo. He mea maikaʻi loa kēia mana i nā laʻana paʻakikī kahi e paʻakikī ai ka loaʻa ʻana o ka hōʻailona.

4  Paʻa a me ka hilinaʻi

Ua ʻike ʻia ka lōʻihi a me ka lōʻihi o ke kukui 365nm Led. ʻAʻole like me nā kumu kukui maʻamau, e hoʻohaʻahaʻa koke i ka manawa, hoʻohiki lākou i ka hana mau a hoʻemi i ka pono o nā pani pinepine. ʻO nā noi lōʻihi e like me ka nānā ʻana i ke kaiapuni mau a i ʻole nā ​​hana ʻoihana automated e pili ana i kēia hilinaʻi.  

Nā noi o 365nm UV LED

ʻO kēia kekahi mau wahi koʻikoʻi kahi e pono ai kēia mau LED:

1  ʻO ke kiʻi ʻoniʻoni ʻo Fluorescence

Ke noiʻi a ʻike ʻo Fluorescence microscopy i ka hoʻolālā cellular a me nā molekala olaola e hoʻohana ana i nā kukui 365nm ka nui. ʻO kā lākou nalu pololei e hiki ai i kekahi ke hoʻohana i kekahi mau fluorophores, e hana ana i nā kiʻi koi a me nā kikoʻī e pono ai no ka noiʻi biomedical a me ka maʻi. Nānā ʻO Tianhui UV LED  nā huahana no nā LED kiʻekiʻe e kūpono i ka microscopy.

2  DNA a me ka Protein Analysis

Hoʻohana ka molecular biology i 365 nm LEDs e hoʻoulu i nā mea kala fluorescence i hoʻopaʻa ʻia i ka DNA a i ʻole nā ​​protein. Aia nā ʻano hana i ka electrophoresis gel a me nā microarrays, kahi e koi ʻia ai ka ʻike biomolecule a me ka helu ʻana, ma muli o ia.

3  ʻIkepili Forensic

ʻO 365 nm ke kū nei no nā LED, pono nui no ka hoʻokolokolo forensic i ka ʻike ʻana i nā ʻano olaola.—e like me ke koko a i ʻole nā ​​wai physiological ʻē aʻe—ʻo ia ka uila ma lalo o ke kukui UV. Hoʻomaʻamaʻa ia i ka ʻimi ʻana a me ka nānā ʻana i nā hōʻike ma nā wahi hewa—ʻoiai ma nā kūlana koi.

4  Nānā Kaiapuni

Hoʻohana ka nānā ʻana i ke kaiapuni i ka 365nm UV LED e ʻālohilohi ma lalo o nā kukui UV a me ka ʻike pollution. Hoʻopuka ia i ka hoʻomalu ʻana i ka maikaʻi o ka wai, ka ʻike kemika, a me ka nānā ʻana i nā kahe ʻaila. Ma muli o ko lākou hilinaʻi, ʻike i nā kūlana climatic he nui, a me ka lōʻihi, kūpono nā kukui Led 365nm no kēia mau kumu.

Mechanisms Ma hope o 365nm LEDs

ʻO ka hoʻonui ʻana i nā LED 365 nm pono e hoʻomaopopo i kā lākou kumu kumu. Aia ma waena o kēia mau hiʻohiʻona koʻikoʻi:

·  Emission Spectrum

Hōʻike nā LED 365 nm i ka hauʻoli kiʻekiʻe o nā fluorophores like ʻole a hoʻokuʻu i ka mālamalama i kekahi lōʻihi nalu. Loaʻa ka fluorescence maikaʻi loa mai ka hoʻololi ʻana i ka ikehu kūpono i hōʻoia ʻia e kēia kiko kikoʻī. ʻO ka hoʻohaʻahaʻa ʻana mai nā lōʻihi nalu ʻē aʻe e ka pūʻulu hoʻokuʻu palena ʻia e kōkua i ka wehewehe ʻana i ka hōʻailona fluorescence.

·  ʻO ka ikaika māmā a me ka nānā

ʻO ka ikaika o ka māmā māmā o 365 nm LED e hōʻoiaʻiʻo i ka hoʻōla maikaʻi ʻana o nā fluorophores a me ke komo hohonu, ʻoiai i loko o nā mea paʻa a mānoanoa paha. Eia kekahi, ma ka microscopy a me nā noi kiʻi ʻē aʻe, nā kukui maʻamau i loaʻa i kēia mau mea Donmake ʻai ʻia ohawah he pomaikai.

·  Hooponopono Wela

Ma ke kōkua ʻana i ka hōʻemi ʻana i ka wela wela a me nā ʻenehana hoʻokele wela ma 365 nm, kōkua nā LED i ka pale ʻana i ka hōʻino ʻana o LED a pili i ka hana. ʻO ka hoʻokuʻu wela kūpono e hōʻoiaʻiʻo i ka hana māmā a hoʻomaikaʻi i ke ola o ka LED, e hōʻoiaʻiʻo ana i ka hilinaʻi no ka hoʻohana lōʻihi i nā noi noi.  

ʻO nā pono ma mua o nā kumu kukui ʻē aʻe

ʻO nā pono mua i helu ʻia ma ʻaneʻi e kōkua e wehewehe i ke kumu e makemake ai kekahi i nā noi fluorescence:

·  Huakaʻi Wavelōʻihi Kūikawā

ʻAʻole e like me nā kumu kukui maʻamau, e like me nā kukui mercury, 365 nm UV LEDs e hoʻohua i ka mālamalama i kahi lōʻihi nalu i kuhikuhi ʻia me ka ʻole o nā kānana hou aʻe. Hāʻawi ia i kahi ala maopopo a maikaʻi hoʻi no ka excitation maikaʻi loa a hoʻomaʻamaʻa i nā noi fluorescence, hoʻomaikaʻi i ka pono. E ʻike i nā huahana Tianhui LED  hāʻawi no nā LED 365nm premium i hana ʻia no kekahi mau hoʻohana.

·  Hoʻoikaika ikaika

ʻO kahi koho ʻoi aku ka maikaʻi o ka ikehu mai ka 365 nm LED e hoʻopau i ka uila ma mua o nā kumu kukui maʻamau. Maikaʻi a aloha i ke kaiapuni, hoʻemi ia i ka wāwae ikehu holoʻokoʻa o nā mea hoʻohana fluorescent.

·  Nui Paʻi

Mai nā lako hana hoʻokolohua paʻakikī a hiki i nā mea hana forensic portable, ʻo ka liʻiliʻi liʻiliʻi o 365nm Led kukui e hiki ai iā lākou ke hoʻokomo i loko o nā mea hana a me nā ʻōnaehana. ʻAʻole pili ko lākou wāwae haʻahaʻa i ka hana; no laila, hiki ke hoʻololi ʻia i nā noi e pili ana i ka fluorescence.

ʻeaohaohaohao:

I ka hopena, ma muli o ko lākou naʻau nui, lōʻihi, a me nā ʻano hauʻoli maikaʻi loa, pono ʻo 365nm UV LED no ka hoʻohana ʻana i ka fluorescence. ʻOi aku ka maikaʻi ma mua o nā kumu kukui maʻamau ma ke ʻano o ka puka hawewe lōʻihi a me ka hoʻokele waiwai.

Hiki i kā lākou ʻano liʻiliʻi ke hoʻohui maʻalahi i nā mea hana a me nā ʻōnaehana. Konohaohaohao UV LED diode Hāʻawi i kahi kikoʻī piha o nā hoʻonā paʻakikī e hoʻokō i kāu mau pono āpau no nā huahana 365 nm LED premium i mālama ʻia no nā hoʻohana like ʻole.