Kutheni i-365nm ye-LED ibalulekile kwizicelo ezisebenzayo zeFluorescence?

Ukusetyenziswa kweFluorescence kuye kwaba ziintsika kwiindawo ezininzi ezahlukeneyo zenzululwazi kunye nezoshishino kuba zibonelela ngokuthe ngqo ukufumanisa i-molecular and visualization. Nokuba umntu uphanda iimfihlo zebhayoloji yeselula okanye ufumana ubungqina obufihlakeleyo bophando, umgangatho womthombo wokukhanya osetyenzisiweyo umisela ukusebenza koku kusetyenziswa.

Ngoku, faka i-365nm LED, utshintsho oluguquguqukayo kwitekhnoloji ye-fluorescence. Ezi LED zivelisa ukukhanya kwi-wavelength efanelekileyo yokuvuselela i-spectrum ebanzi ye-fluorophores, ukuvelisa imifanekiso ecacileyo kunye neenkcukacha ezichanekileyo.

Nangona kunjalo, yintoni eyahlula i-365 nm UV LED phakathi kolwandle lwemithombo yokukhanya? Eli nqaku liza kuphanda ngezibonelelo zabo ezininzi kwaye lixoxe ngokuba kutheni ziyimfuneko ekusetyenzisweni kwe-fluorescence. Tianhui UV LED ngumthombo omkhulu wepremium 365nm UV iimveliso ze-LED.

Yintoni iFluorescence

I-Fluorescence yinkqubo apho ezinye iimolekyuli zifunxa ukukhanya kubude bobude obunye zize zikukhuphe kwenye, ngokufuthi kubude obude. Lo mgangatho usetyenziswa kakhulu ukujonga, ukuchonga, kunye nokulinganisa iimolekyuli ezithile kwiindawo zenzululwazi, ezonyango, kunye nezoshishino.

Ukujongwa kokusingqongileyo, uhlalutyo lweekhemikhali, kunye ne-biological imaging izuza kakhulu kwi-fluorescence. Ibhaqa amanqanaba emizuzu emincinci yamachiza kwiisampulu ezintsonkothileyo, ebonelela ngobuntununtunu obukhulu kunye neenkcukacha. Ukukhutshwa kokukhanya okuchanekileyo kwe-fluorophores kuvumela abaphandi ukuba bahlole iiseli, iityhefu zamabala, kunye nokuvavanya ngokuchanekileyo iisampulu zebhayoloji.

Indima ye-365nm LEDs

Ukusetyenziswa kwi-fluorescence kuxhomekeke kakhulu kwi-365 nm Led light. Ezi ziindima abazinikezelayo, ngoko ke zibalulekile kwiinkalo ezininzi.

1  Elona Excitation for Fluorophores

Ukonwabela ii-fluorophoreson ezininzi eziqhelekileyo, i-365nm ye-UV ye-UV ivelisa ukukhanya kwe-UV kubude obuluncedo. Olu bude bobude bulungele ukuvelisa ezi molekyuli ukuba zikhanyise, zivelise umqondiso onamandla kunye ocacileyo. Ekubeni zibonelela ngamandla okuvuselela afunekayo ukunyusa amandla e-fluorescence, ii-LED ze-365 nm ziphezulu kuneminye imithombo yokukhanya, kubandakanywa ne-fluorescence microscopy kunye ne-spectroscopy.

2  Uvakalelo oluPhezulu kunye neSigqibo

Ukuqina okukhulu kunye nokuzinza kwe-365 nm LEDs kwenza ukusetyenziswa kwe-fluorescence ngovakalelo kunye nesisombululo. Izifundo zixhomekeke kwiziphumo ezichanekileyo neziphindaphindwayo, ezixhomekeke ekuphumeni kokukhanya rhoqo, ke ezi LED zibalulekile.

Kwizicwangciso zophando kunye nokuxilongwa, kunye nakwi-imaging ebanzi, zifanelekile kwimilinganiselo echanekileyo. Ii-LED zeTianhui UV iimveliso zenzelwe ukwanelisa eyona nqobo ilungileyo yokuchaneka kunye nomgangatho kwabo bafuna ubucukubhede Isisombululo se-UV LED

3  Uphuculo loMqondiso ukuya kwiNgxolo

Kwizicelo ze-fluorescence, ukwahlula isignali ye-fluorescence kwingxolo yangasemva ixhomekeke kuphawu oluphezulu ukuya kumlinganiselo. I-365nm ye-UV ye-UV inika umthombo onamandla wokuvuselela ovelisa uphawu olucacileyo nolucacileyo lwe-fluorescence. Esi sixhobo siluncedo kakhulu kwiisampulu ezintsonkothileyo apho kunokuba ngumngeni ukufumana isignali ekujoliswe kuyo.

4  Ukuqina kunye nokuthembeka

Kuyaziwa ukuba ubude kunye nobude be-365nm Led ukukhanya. Ngokungafani nemithombo yokukhanya eqhelekileyo, ehla ngokukhawuleza ekuhambeni kwexesha, ithembisa ukusebenza rhoqo kunye nokunciphisa imfuno yokutshintshwa rhoqo. Izicelo zexesha elide ezinjengokubeka iliso kokusingqongileyo okuqhubekayo okanye imisebenzi yoshishino oluzenzekelayo ixhomekeke koku kuthembeka.  

Ukusetyenziswa kwe-365nm UV LED

Ezi zezinye iindawo ezibalulekileyo apho ezi LED zibalulekile:

1  I-Fluorescence Microscopy

I-microscopy ye-Fluorescence iphanda kwaye ijonge ulwakhiwo lweselula kunye neemolekyuli zebhayoloji zisebenzisa izibane ezingama-365nm ubukhulu becala. Ubude bawo obuchanekileyo buvumela umntu ukuba asebenzise i-fluorophores eliqela, evelisa imifanekiso ebukhali neneenkcukacha ezifunekayo kufundo lwebhayoloji kunye noxilongo. Jonga Ii-LED zeTianhui UV  iimveliso ze-LED ezisebenza kakhulu zilungele i-microscope.

2  Uhlalutyo lwe-DNA kunye neProtheyini

Ibhayoloji yemolekyuli isebenzisa ii-LED ezingama-365 nm ukwenza idayi ye-fluorescence ebotshelelwe kwi-DNA okanye kwiiprotheni. Iindlela ziquka i-gel electrophoresis kunye ne-microarrays, apho ukuchongwa kwe-biomolecule echanekileyo kunye nobungakanani bufunekayo, kuxhomekeke kuyo.

3  Uhlalutyo lweNkundla

I-365 nm imele ii-LEDs, eziyimfuneko kuphando lwasenkundleni ekuqapheliseni umkhondo webhayoloji.—njengegazi okanye olunye ulwelo lomzimba—edanyaza phantsi kokukhanya kwe-UV. Ifezekisa ukukhangelwa kunye nohlalutyo lobungqina kwiindawo zolwaphulo-mthetho—nakwiimeko ezinzima.

4  Ukubeka iliso kokusiNgqongileyo

Ukubeka iliso kokusingqongileyo kusebenzisa i-365nm ye-UV ye-UV ukuze ikhanyise phantsi kwe-UV ye-toxins yokukhanya kunye nokuchongwa kongcoliseko. Ijongana nolawulo lomgangatho wamanzi, ukubonwa kweekhemikhali, kunye nokulandelela ukuchitheka kweoyile. Ngenxa yokuxhomekeka kwabo, ubuntununtunu kwiimeko ezininzi zemozulu, kunye nokuqina, izibane ze-LED ze-365nm zilungele ezi njongo.

Iinkqubo ezingemva kwee-LED ze-365nm

Ukuphucula i-365 nm LEDs kufuna ukuqonda imigaqo yazo esisiseko. Phakathi kwezi kukho iimpawu ezibalaseleyo:

·  I-Spectrum yokukhupha

Ii-LED ze-365 nm zibonisa uchulumanco oluphezulu lwee-fluorophore ezahlukeneyo kwaye zikhupha ukukhanya kubude obuthile. Eyona fluorescence ifumaneka kunikezelo lwamandla olusebenzayo oluqinisekiswa ngulo mbala uthile wokukhupha. Ukunciphisa ukuphazamiseka kwamanye amaza ngamaza ombane ngebhanti yokukhutshwa okulinganiselweyo kunceda ukucacisa isignali ye-fluorescence.

·  Ubunzulu bokukhanya kunye nokuGxininisa

Ukukhanya okuqinileyo kwe-365 nm LEDs kuqinisekisa ukusebenza okusebenzayo kwe-fluorophores kunye nokungena nzulu, nakwizinto ezishinyeneyo okanye ezishinyeneyo. Ngaphezu koko, kwi-microscope kunye nezinye izicelo zokucinga, ukukhanya okulungiselelweyo okwenziwe kufumaneke zezi Imodyuli ye-UV LED iluncedo.

·  Ulawulo lobushushu

Ngokunceda ekunciphiseni ubushushu obugqithisileyo kunye nobuchwepheshe obuphambili bolawulo lobushushu kwi-365 nm, ii-LED zinceda ukuthintela ukuthotywa kwe-LED kunye nokuchaphazela ukusebenza. Ukutshatyalaliswa kobushushu okuchanekileyo kuqinisekisa imveliso yokukhanya eqhubekayo kwaye iphucula ubomi be-LED, iqinisekisa ukuthembeka kokusetyenziswa ixesha elide kwizicelo ezifunayo.  

Izinto eziluncedo ngaphezu kweminye imithombo yokukhanya

Iinzuzo eziphambili ezidweliswe apha zinceda ukucacisa ukuba kutheni umntu ekhetha usetyenziso oluthile lwe-fluorescence:

·  Isiphumo sobude beWaveleng obuthile

Ngokungafaniyo nemithombo yokukhanya eqhelekileyo, efana nezibane zemercury, ii-365 nm UV ii-LED zivelisa ukukhanya kubude obuchaziweyo ngaphandle kokucoca. Inika indlela ecacileyo kunye nesebenzayo yokuvuselela ngokufanelekileyo kwaye yenza lula izicelo ze-fluorescence, ukuphucula ukusebenza kakuhle. Fumana iimveliso Tianhui LED  inikeza iprimiyamu 365nm LEDs ezenzelwe ukusetyenziswa okuthile.

·  Ukusebenza kakuhle kwamandla

Enye indlela eyonga amandla kuba ii-LED ze-365 nm zisebenzisa umbane omncinci kakhulu kunemithombo yokukhanya eqhelekileyo. Ixabiso eliphantsi kwaye linobubele kokusingqongileyo, linciphisa unyawo lwamandla onke lwabasebenzisi be-fluorescent.

·  Ubungakanani obubambeneyo

Ukusuka kwizixhobo ezintsonkothileyo zaselabhoratri ukuya kwizixhobo eziphathwayo zeforensic, ubungakanani obuncinci be-365nm Led light ibavumela ukuba babandakanywe kwizixhobo ezininzi kunye neenkqubo. Unyawo lwabo oluthobekileyo aluchaphazeli ukusebenza; kungoko, ziyakwazi ukuziqhelanisa nosetyenziso oluninzi olusekwe kwi-fluorescence.

Ukugqitywa:

Ukuqukumbela, ngenxa yobuntununtunu babo obukhulu, ubomi obude, kunye neempawu ezibalaseleyo zolonwabo, i-365nm ye-UV LED iyimfuneko ekusetyenzisweni kwe-fluorescence. Bangcono kunemithombo yokukhanya eqhelekileyo ngokubhekiselele kwimveliso ye-wavelength echanekileyo kunye noqoqosho lwamandla.

Ifomu yabo encinci yenza ukudibanisa okuguquguqukayo kwizixhobo ezininzi kunye neenkqubo ezinokwenzeka. TianhuiName UV LED idiode inikeza uluhlu olupheleleyo lwezisombululo eziphucukileyo zokwanelisa zonke iimfuno zakho zeprimiyamu ye-365 nm yeemveliso ze-LED ezilungiselelwe ukusetyenziswa okwahlukeneyo.