Hobaneng ha 365nm LED e le Bohlokoa Bakeng sa Likopo tse Atlehang tsa Fluorescence?

Lisebelisoa tsa Fluorescence li fetohile litšiea libakeng tse ngata tse fapaneng tsa saense le indasteri kaha li fana ka pono e nepahetseng ea limolek'hule le pono. Hore na motho o ntse a batlisisa makunutu a biology ea cellular kapa o fumana bopaki bo patehileng ba forensic, boleng ba mohloli oa leseli o sebelisitsoeng ke ona o lekanyetsang katleho ea ts'ebeliso ena.

Joale, kenya 365nm LED, phetoho ea phetoho ho theknoloji ea fluorescence. Li-LED tsena li hlahisa leseli ka bolelele bo loketseng bakeng sa ho susumetsa mefuta e mengata ea fluorophores, ho hlahisa litšoantšo tse hlakileng haholoanyane le boitsebiso bo nepahetseng haholoanyane.

Leha ho le joalo, ke eng e khethollang 365 nm UV LED har'a leoatle la mehloli ea leseli? Sengoliloeng sena se tla etsa lipatlisiso ka melemo ea bona e 'maloa le ho tšohla hore na ke hobane'ng ha e le tsa bohlokoa bakeng sa tšebeliso ea fluorescence. Tianhui UV LED ke mohloli o motle oa lihlahisoa tsa LED tsa premium 365nm UV.

Fluorescence ke eng?

Fluorescence ke mokhoa oo ka oona limolek'hule tse ling li monyang khanya sebakeng se seng sa leqhubu ebe li le ntša ho le leng, hangata ka bolelele bo bolelele ba maqhubu. Boleng bona bo sebelisoa haholo ho sheba, ho khetholla, le ho lekanya limolek'hule tse itseng likarolong tsa saense, tsa bongaka le tsa indasteri.

Tlhokomelo ea tikoloho, tlhahlobo ea lik'hemik'hale, le litšoantšo tsa baeloji li rua molemo haholo ho fluorescence. E lemoha esita le metsotso e seng mekae ea lithethefatsi ka lisampole tse rarahaneng, e fana ka kutloisiso e kholo le ho hlaka. Ho ntšoa ha leseli le hlakileng la fluorophores ho etsa hore bafuputsi ba hlahlobe lisele, chefo ea matheba, le ho lekola lisampole tsa tlhaho ka nepo.

Karolo ea 365nm LEDs

Lisebelisoa tsa fluorescence li itšetlehile haholo ka leseli la 365 nm Led. Tsena ke likarolo tseo ba fanang ka tsona, ka hona li bohlokoa mafapheng a mangata.

1  Ntlafatso e nepahetseng bakeng sa Fluorophores

Bakeng sa thabo ea li-fluorophoreson tse ngata tse tloaelehileng, 365nm UV LED e hlahisa leseli la UV ka bolelele bo sebetsang haholo. Bolelele bona ba maqhubu bo loketse ho hlahisa limolek'hule tsena hore li khanye, ho hlahisa pontšo e matla le e hlakileng. Kaha li fana ka matla a hlasimollang a hlokahalang ho eketsa matla a fluorescence, li-LED tsa 365 nm li phahametse mehloli e meng ea leseli, ho kenyelletsa le microscopy ea fluorescence le spectroscopy.

2  Kutloelo-bohloko e Phahameng le Qeto

Matla a maholo le botsitso ba 365 nm LEDs li nolofalletsa tšebeliso ea fluorescence ka kutloisiso le tharollo. Boithuto bo ipapisitse le liphetho tse nepahetseng le tse ka phetoang, tse itšetlehileng ka tlhahiso ea khanya e sa feleng, kahoo li-LED tsena li bohlokoa.

Litlhophisong tsa lipatlisiso le tlhahlobo, hammoho le litšoantšong tse felletseng, li nepahetse bakeng sa litekanyo tse nepahetseng. Li-LED tsa Tianhui UV lihlahisoa li entsoe ho khotsofatsa litekanyetso tse ntle ka ho fetisisa tsa ho nepahala le boleng bakeng sa ba batlang tse rarahaneng Litharollo tsa UV LED

3  Karolelano e ntlafetseng ea Pontšo-ho-Lerata

Lits'ebetsong tsa fluorescence, ho khetholla lets'oao la fluorescence ho tsoa lerata le ka morao ho ipapisitse le karo-karolelano e phahameng ea lets'oao. 365nm UV LED e fana ka mohloli o matla oa ho hlasimolla o hlahisang lets'oao le hlakileng le hlakileng la fluorescence. Bokhoni bona bo bohlokoa haholo ho lisampole tse rarahaneng moo ho ka bang thata ho fumana lets'oao la sepheo.

4  Ho tšoarella le ho Tšepahala

Hoa tsebahala hore lebone la 365nm Led le bolelele hakae ebile le tšoarella. Ho fapana le mehloli e tloaelehileng ea leseli, e senyang kapele ha nako e ntse e feta, e tšepisa ts'ebetso e sa khaotseng le ho fokotsa tlhokahalo ea ho nkeloa sebaka khafetsa. Likopo tsa nako e telele joalo ka tlhahlobo e tsoelang pele ea tikoloho kapa ts'ebetso ea indasteri e ikemetseng e itšetlehile ka ts'epo ena.  

Lisebelisoa tsa 365nm UV LED

Tsena ke libaka tse ling tsa bohlokoa moo li-LED tsena li leng bohlokoa:

1  Fluorescence Microscopy

Fluorescence microscopy e etsa lipatlisiso le ho bona meralo ea lisele le limolek'hule tsa baeloji tse sebelisang mabone a 365nm haholo. Bophahamo ba bona bo nepahetseng bo lumella motho ho sebelisa li-fluorophore tse 'maloa, ho hlahisa litšoantšo tse hlakileng le tse qaqileng tse hlokahalang bakeng sa boithuto ba biomedical le tlhahlobo. Sheba Li-LED tsa Tianhui UV  lihlahisoa tsa li-LED tse sebetsang hantle tse loketseng microscope.

2  Tlhahlobo ea DNA le Liprotheine

Biology ea limolek'hule e sebelisa li-LED tsa 365 nm ho kenya lidae tsa fluorescence tse hokahaneng le DNA kapa liprotheine. Mekhoa e kenyelletsa li-electrophoresis tsa gel le li-microarrays, moo ho hlokahalang ho tsebahatsa biomolecule le quantification, ho itšetlehile ka eona.

3  Tlhahlobo ea Forensic

365 nm e emetse li-LED, tse hlokahalang bakeng sa lipatlisiso tsa forensic mabapi le ho lemoha mesaletsa ea tlhaho.—joalo ka madi kapa maro a mang a mmele—e phatsimang ka tlas'a lebone la UV. E nolofatsa patlo le tlhahlobo ea bopaki libakeng tsa botlokotsebe—esita le maemong a batlang.

4  Tlhokomelo ea Tikoloho

Tlhokomelo ea tikoloho e sebelisa 365nm UV LED ho khantša tlas'a chefo e khanyang ea UV le boitsebiso ba tšilafalo. E sebetsana le taolo ea boleng ba metsi, ho lemoha lik'hemik'hale, le ho lateloa ha oli e tšolohileng. Ka lebaka la ts'epahalo ea bona, kutloelo-bohloko maemong a mangata a leholimo, le ho tšoarella nako e telele, mabone a 365nm Led a loketse merero ena.

Mekhoa e ka morao ho 365nm LEDs

Ho ntlafatsa li-LED tsa 365 nm ho hloka kutloisiso ea melao-motheo ea bona. Har'a tsena ho na le litšobotsi tse hlahelletseng:

·  Emission Spectrum

Li-LED tsa 365 nm li bonahatsa tlhōrō ea tlhōrō ea li-fluorophore tse fapaneng 'me li ntša khanya ka bolelele bo itseng ba maqhubu. Fluorescence e ntle ka ho fetisisa e fumanoa ho tsoa phetisong e sebetsang ea matla e netefalitsoeng ke sebono sena sa tlhahiso. Ho fokotsa ho kena-kenana le maqhubu a mang ka sehlopha se lekanyelitsoeng sa emission ho thusa ho hlakisa lets'oao la fluorescence.

·  Matla a Leseli le Tsepamiso

Matla a khanya a matla a 365 nm LED a tiisa ts'ebetso e sebetsang ea fluorophores le ho kenella ka botebo, esita le ka thepa e teteaneng kapa e teteaneng. Ho feta moo, ho microscope le lits'ebetso tse ling tsa ho nka litšoantšo, mabone a ikhethileng a fumanehang ke tsena Module oa LED oa UV e molemo.

·  Taolo ea Mocheso

Ka ho thusa ho fokotsa mocheso o feteletseng le theknoloji e tsoetseng pele ea ho laola mocheso ka 365 nm, li-LED li thusa ho thibela ho senyeha ha LED le ho ama ts'ebetso. Phallo e nepahetseng ea mocheso e netefatsa tlhahiso e tsoelang pele ea mabone le ho ntlafatsa bophelo ba LED, ho netefatsa ts'epahalo ea ts'ebeliso ea nako e telele lits'ebetsong tse hlokahalang.  

Melemo Ho Feta Mehloli e Meng ea Leseli

Melemo ea mantlha e thathamisitsoeng mona e thusa ho hlalosa hore na ke hobaneng ha motho a rata lisebelisoa tse itseng tsa fluorescence:

·  Sehlahisoa se khethehileng sa Wavelength

Ho fapana le mehloli e tloaelehileng ea leseli, joalo ka mabone a mercury, li-LED tsa 365 nm tsa UV li hlahisa leseli ka bolelele bo boletsoeng ntle le li-filters tse ling. E fana ka mokhoa o hlakileng le o sebetsang oa ho hlasimolla hantle le ho nolofatsa lits'ebetso tsa fluorescence, ho ntlafatsa ts'ebetso. Fumana lihlahisoa Tianhui LED  e fana ka li-LED tsa premium 365nm tse etselitsoeng tšebeliso e itseng.

·  Matla a Matla

Mokhoa o mong o sebetsang hantle ho tloha ha li-LED tsa 365 nm li sebelisa motlakase o fokolang haholo ho feta mehloli e tloaelehileng ea mabone. E theko e tlaase ebile e le botsoalle tikolohong, e fokotsa matla a matla a basebelisi ba fluorescent.

·  Boholo bo Compact

Ho tloha ho lisebelisoa tse rarahaneng tsa laboratori ho isa ho lisebelisoa tse nkehang tsa forensic, boholo bo bonyenyane ba 365nm Led lebone bo li lumella ho kenyelletsoa lisebelisoa le lits'ebetso tse ngata. Boemo ba bona bo itekanetseng ha bo ame ts'ebetso; ka hona, li khona ho ikamahanya le lisebelisoa tse ngata tse thehiloeng ho fluorescence.

Qetello:

Qetellong, ka lebaka la kutlo ea bona e kholo, bophelo bo bolelele, le litšoaneleho tse ntle ka ho fetisisa tsa ho hlasimolla, 365nm UV LED ke ea bohlokoa haholo bakeng sa ts'ebeliso ea fluorescence. Li molemo ho feta mehloli e tloaelehileng ea leseli mabapi le tlhahiso e nepahetseng ea maqhubu a maqhubu le moruo oa matla.

Sebopeho sa bona se senyenyane se boetse se etsa hore ho kopanngoa ha maemo ho lisebelisoa tse ngata le mekhoa e khonehe. Tianhui UV LED diode e fana ka litharollo tse ngata tse rarahaneng ho khotsofatsa litlhoko tsohle tsa hau bakeng sa lihlahisoa tsa LED tsa premium 365 nm tse etselitsoeng litšebeliso tse fapaneng.